However, there are many causes for decreased fertility, such as genetic causes, which can seriously affect the system. Proper reproductive management is essential to achieve optimal rates in a cattle production system. Manual method was recommended only for analysis of normal or abnormal number of chromosome. It was recommended to performed chromosomal investigation of breeding bulls using advanced sophisticated tools of analysis like cytovision image analysis of fluorescent technique. The karyotype analysis of all cattle showed that the chromosomes of one cell and different individual each breed varied in size, shape and position of centromere. It was observed that all cattle tested in this research were normal categories. It showed that the 2 N diploid number of chromosome was normal was 60, there were 58 autosomes and 2 sex chromosomes in all cattle observed. Result of both method of karyotyping may accepted for analysing method of abnormal/normal chromosome. Slides were examined under high phase-contrast microscope, chromosome analysis using cytovision software and manual analysis straightly captured under microscope then arranged. Slides were prepared and dried then stained with Giemsa. Colcichine was added after 70 hours and kept for 2–3 hours.

Sample was added to medium (Karyo MAX Gibco) then placed in CO2 incubator at 38☌. Method performed by collecting blood samples from first generation of crossing breed Madura Cattle vs. A number of cattle breeds have been reported on of the 50 exotic breeds with the problem of 1/29 translocation, then the chromosome analysis to be important to execute. In Indonesia, AI implementation in cattle have been started intensively, especially using imported bulls (bos Taurus) i.e. Chromosomal abnormalities are usually considered to be a plague and are to eliminate. On the basis of the important chromosome abnormalities and their negative effect in the near future, chromosomal investigation of breeding bull especially for Artificial Insemination (AI) began in different countries. Chromosome analysis or karyotyping is one among powerful methods to characterize normal or abnormal genetic of animals.

Cytovision software manual